FIGURE 3:

Oocyte entry during ovulation is required for alignment of spermathecal actin bundles. (A) Quantification of actin bundle anisotropy (degree of alignment) using FibrilTool. Each point represents a single cell, and no more than three cells were measured from the same animal. For each condition, from left to right, 44, 63, 152, 42, 27, 35, 54, and 49 cells. (B) Representative phalloidin staining results for each condition in A. Yellow line indicates a single cell selected for measurement with FibrilTool, and numbers indicate anisotropy measured for each cell. Error bars represent SEM. Unpaired t test: ns, p > 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001. Scale bar, 5 μm.