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. 2017 Jul 7;28(14):1937–1949. doi: 10.1091/mbc.E17-01-0029

FIGURE 4:

FIGURE 4:

Actin bundle alignment is proportional to cell stretch. (A, B) Confocal maximum intensity projections showing spermathecal actin labeled with GFP (GFP::ACT-1) in a slightly stretched spermatheca containing an oocyte fragment (A) and normally stretched spermatheca containing the entire oocyte (B) at ∼20 min after oocyte entry. (A’, B’) A central, sagittal z-slice from A and B. The yellow dashed line indicates measurement of spermathecal cross-sectional area. (C) Comparison of spermathecal cross-sectional area and actin network anisotropy (degree of alignment) achieved ∼20 min after oocyte entry, depicting a positive relationship between spermathecal cell stretch and actin network anisotropy. Two cells were measured from nine animals (18 cells). Black line: best-fit linear regression. Scale bar, 20 μm.