Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 7;28(14):1937–1949. doi: 10.1091/mbc.E17-01-0029

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Wirshing and Cram. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 5: — Actin bundle alignment coincides with cell contraction during ovulation. (A) Maximum intensity projections from a 4D confocal ovulation movie of spermathecal cells expressing GFP-labeled actin (GFP::ACT-1). At 100 s after oocyte entry, spermathecal cells are stretched, yet display the webby preovulation actomyosin network. At 750 s after oocyte entry, contracting cells show development of parallel actomyosin bundles. At 1500 s after oocyte entry, spermathecal cells exhibit parallel actomyosin bundles oriented along the long axis of each cell. (B) Quantification of cell contraction and actomyosin bundle anisotropy (degree of alignment) in two cells from independent ovulation movies. FibrilTool was used to quantify actin anisotropy in individual cells (yellow outline in A). Cell shortening coincides with a rapid increase in actin anisotropy ∼500 s after oocyte entry. Scale bar, 20 μm.