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Evidence for RRP6L1 trimming of Pol V transcript 3′ ends. Chop-PCR and 3′ RACE results for Pol V transcribed loci: A. IGN5A, B. IGN25, C. IGN23, D. IGN35. Chop PCR was conducted with the enzymes HaeIII or AluI, both of which are inhibited by CHH methylation. Box plots show 3′ end lengths in basepairs, measured from the internal gene specific primer used in the 3′ RACE reactions to the ends of the RNAs. See also Figures S6–S7 and Table S4.
