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. Author manuscript; available in PMC: 2017 Aug 3.
Published in final edited form as: Cell Rep. 2017 Jun 27;19(13):2796–2808. doi: 10.1016/j.celrep.2017.05.091

Figure 7.

Figure 7

Model for CTD-mediated coordination of AGO4 transcript cleavage and RRP6L1 engagement of cleaved RNA 3′ ends. AGO4 and RRP6L1 bind adjacent subdomains of the NRPE1 CTD, such that co-transcriptional slicing of Pol V transcripts by AGO4, guided by basepaired 24 nt siRNAs, may be coupled to RRP6L1 engagement of cleaved RNA 3′ ends. RRP6L1’s trimming of Pol V transcripts, with pausing at sites of secondary structure, may facilitate RNA retention, allowing Pol V transcript-binding proteins, such as the IDP complex, to recruit the de novo cytosine methyltransferase, DRM2 to cleaved RNAs while Pol V transcription of nascent RNA continues.