Table 2.
Yolk sac hematopoiesis in SOCS3−/− mice
| E10.5
|
E11.5
|
|||||
|---|---|---|---|---|---|---|
| SOCS3+/+ | SOCS3+/− | SOCS3−/− | SOCS3+/+ | SOCS3+/− | SOCS3−/− | |
| Number analyzed | 3 | 2 | 2 | 10 | 19 | 3 |
| Cellularity, ×10−6 | 0.24 ± 0.15 | 0.14 ± 0.01 | 0.18 ± 0.15 | 0.35 ± 0.15 | 0.33 ± 0.16 | 0.20 ± 0.22 |
| Myeloid-CFC, per 1 × 104 | 7 ± 3 | 9.5 ± 5 | 10.5 ± 1 | 6 ± 2 | 4 ± 2 | 5 ± 1 |
| BFU-E, per 1 × 104 | 12 ± 5 | 23 ± 24 | 29 ± 5 | 7 ± 3 | 5 ± 3 | 18 ± 15 |
| Mix-CFC, per 1 × 104 | 4 ± 1 | 4 ± 5 | 4 ± 3 | 2 ± 1.5 | 1 ± 1 | 1 ± 1 |
Results represent the mean ± SD of data from multiple embryos. Triplicate cultures were stimulated with SCF, IL-3, and EPO and incubated for 7 days in a humidified atmosphere of 5% CO2/air at 37°C.