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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Virology. 2017 Sep;509:98–111. doi: 10.1016/j.virol.2017.06.010

Figure 2. ECTV exhibits delayed plaque formation and genome replication relative to VACV.

Figure 2

(A) Confluent monolayers of BS-C-1 cells were initially infected at a low MOI (~0.001) to allow for proper visualization of plaque development over time. The otherwise wild-type viruses were engineered to express GFP. The images were acquired using an epifluorescence microscope. All images are at 4× total magnification and representative of ECTV and VACV plaques at each time point. (B) A representative image showing the presence of cytoplasmic virus factories visualized by DAPI staining. Cell nuclei are labeled as “N” in the image, which is at 1,000× total magnification. (C) BS-C-1 cells were infected (MOI=10) with either ECTV or VACV for various lengths of time. For each time point, 150 randomly viewed cells within each infection condition were scored as either positive or negative for the presence of a virus factory of any size. The bars depict the average values and the error bars represent the standard deviations of two independent experiments. N.D., not determined.