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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Virology. 2017 Sep;509:98–111. doi: 10.1016/j.virol.2017.06.010

Figure 6. RNase L activation is observed only in VACV-infected cells at late time points.

Figure 6

(A) BS-C-1 or BS-C-1+E3L cells were infected (MOI=5) with either ECTV or VACV. Total RNA was isolated at the indicated time points and separated using a 1% bleach TBE gel. Each lane contained 4 µg of RNA. The data are representative of three independent trials. (B) BS-C-1 cells were infected (MOI=5) with VACV either in the presence of absence of AraC. RNA breakdown was detected as above at 18 hrs. post-infection. (C) BS-C-1 or BS-C-1+E3L cells were infected (MOI=5) with VACVΔE3LΔK3L. RNA breakdown was detected as above at the indicated time points. (D) BS-C-1 or BS-C-1+E3L cells were infected (MOI=10) with VACVΔE3LΔK3L for 18 hrs. prior to staining for dsRNA. The flow cytometric data are depicted in histogram format and representative of two independent trials.