Table 1.
Summary of gene sizes and oligonucleotides used to produce RNA probes for Northern blot analysis.
| Target gene | Size of ORF (bp) |
Primers | oligonucleotide sequence (5’ to 3’) | product size (bp) |
|---|---|---|---|---|
| MVA/VACV/ECTV 050L/E3L/EVM043 | 573 | E3L-F | TTACTAGGCCCCACTGATTC | 406 |
| E3L-R | GTTCTGACGCAGAGATTGTG | |||
| MVA/VACV/ECTV 078R/G8R/EVM070 | 783 | G8R-F | AATGTAGACTCGACGGATGAG | 484 |
| G8R-R | ACGCATCAGTATTACCAGGAG | |||
| MVA/VACV/ECTV 047R/F17R/EVM040 | 306 | F17R-F | CATTTTGCATCTGCTCATACTC | 262 |
| F17R-R | TAGCCGCGAACATATTTTGTAG | |||
| MVA/VACV/ECTV 094L/H4L/EVM086 | 2,388 | H4L-F | TAGAATTTGCGAGAAGGGATC | 341 |
| H4L-R | GTCACACGTTAGCTCTTTGAAG |