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. 2017 Aug 3;11:447. doi: 10.3389/fnins.2017.00447

Figure 1.

Figure 1

(a) Representative confocal images showing the injection site for the retrograde label—Rhodamine B+ Amine (10,000 MW)—in the Nucleus of tractus solitarius. NTS, Nucleus of tractus solitarius; Sol, Solitary tract (M: Medial, L: Lateral, V: Ventral); SP5I, spinal trigeminal nucleus (interploar part); ml, medial lemniscus; py, pyramidal tract; 4V, fourth ventricle; 10cb, lobule of 10th cerebellar vermis; CVL, caudoventrolateral reticular nucleus; LPGi, lateral paragigantocellular nucleus. (b) Fluorescence images showing the co-localization of Rhodamine and NeuN (neurons) in the posterior and anterior parts of the Sol (scale bar = 50 μm). (c) Confocal images showing the distribution of Rhodamine labelled retrograde vesicles in the PVN after a 7-day survival period (scale bar = 50 μm). (Inset) Localization of Rhodamine in the PVN neurons (scale bar = 30 μm). (d) Co-localization of retrograde labels and neurons (NeuN) in the PVN (scale bar = 20 μm).