Figure 4.

Low-dose sorafenib treatment disturbs the extracellular amino-acid profile of hippocampal cultures. Primary hippocampus cultures were incubated with 5 μM sorafenib (or control) for 24 h and their supernatant was collected and analyzed by high-performance liquid chromatography (HPLC). (a) Amino acids upregulated in response to 5 μM sorafenib treatment with their concentration relative to the respective controls. Asterisks indicate the level of significance in an unpaired two-sided t-test. The bar plot shows means with standard deviations. Number of experiments: n=12 for control, n=10 for 5 μM sorafenib. (b) Amino acids downregulated in response to 5 μM sorafenib treatment with their concentration relative to the respective controls. Asterisks indicate the level of significance in an unpaired two-sided t-test. The bar plot shows means with standard deviations. Number of experiments: n=12 for control, n=10 for 5 μM sorafenib. Levels of significance: **P<0.01, ***P<0.001. A comprehensive table of all descriptive statistics and the analysis parameters can be found in Table 1.