High fat feeding has no effect of PKG Iα disulfide dimerization. (A) Similar left ventricular (LV) mass, stroke volume, cardiac output and posterior wall thickness in WT and KI mice after feeding with obesogenic diet (grey bars) for 27 weeks. (B) Smaller LV systolic and diastolic dimension and volume in WT mice fed an obesogenic diet, compared to the KI. (C) Blood concentration of sodium (Na+), chloride (Cl-) and blood urea nitrogen (BUN) in WT and KI mice after feeding with obesogenic diet (grey bars) for 27 weeks, compared to the normal diet (white bars). (D) Feeding mice with high fat obesogenic diet for 27 weeks did not result in increase of PKG Iα disulfide dimerization in heart. n = 5/group. Data represented as mean ± SEM. *P ≤ 0.05 versus normal diet.). nd – normal diet, od – obesogenic high fat det.