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. 2017 Sep;110:316–331. doi: 10.1016/j.freeradbiomed.2017.06.022

Fig. 6.

Fig. 6

Effects of U46619, LY83583, antioxidants and SrcFK inhibition on RhoA-EmGFP translocation in PASMC. A-D: Fluorescent imaging of representative RhoA-EmGFP transfected PASMC at rest (left panels), in the presence of stimulus (middle panels A-B: U46619, U46, 100 nM or C-D: LY83583, LY, 1 µM) or after washout followed by repeated exposure to stimulus in the presence of either Tempol, PP2 or ebselen (right panels). E-F: Quantification of spot/patch fluorescence intensity expressed as % change above control levels during stimulus ± inhibition (E: U46619, F: LY83583). ##P<0.001 vs. baseline, **P<0.001 vs. U46619, 2-way ANOVA, n=8–11 cells from a total of seven different cell lines. Each measurement is combined from at least 3 spots/patches from each cell. See supplementary Fig. S6 for control responses.