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. Author manuscript; available in PMC: 2017 Aug 3.
Published in final edited form as: Basic Res Cardiol. 2016 Apr 4;111(3):29. doi: 10.1007/s00395-016-0547-4

Fig. 1.

Fig. 1

Evaluation of mitochondrial DNA damage (a) and respiration (NADH dependent) in isolated cardiac mitochondria (b–e). In ZOF, the size of the BamHI digest band was greatly reduced compared to ZLN (a), indicating mtDNA damage, and about a fourfold higher lesion frequency. The respiratory control ratio (RCR) (b) and state 4 respiration (c) were not different among the four groups. Decreased state 3 respiration (d) was shown in ZOF and ZOF + mt-tat-EndoIII compared to ZLN. (e) Maximal respiration during uncoupling with FCCP revealed decreased respiration in ZLN treated with mt-tat-ExoIII (vs. ZLN). Also, treatment of ZOF with mt-tat-EndoIII improved respiration compared to ZOF (P < 0.05). N = 4–5 for all measurements