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. 2017 Apr 1;8(28):45213–45223. doi: 10.18632/oncotarget.16779

Figure 6. MiR-27a antioligonucleotides promote the formation of Apaf-1-caspase-9 complex in TRAIL-treated colorectal CSCs.

Figure 6

(A) After treatment with miR-27a antioligonucleotides, Apaf-1 siRNA and TRAIL, cleavage of caspase-8 was evaluated by western blot analysis in HT29 and SW480 CSCs. (B) Mitochondrial membrane potential (∆ Ψm) was detected by using JC-1 on flow cytometry. (C) After separation of mitochondria, cytochrome c in mitochondria and cytoplasm was detected by western blot analysis. (D) Co-immunoprecipitation and western blot analysis was performed to evaluate the formation of Apaf-1-caspase-9 complex. (E) Cleavage of caspase-9 and caspase-3 in HT29 and SW480 CSCs was evaluated by western blot analysis.