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. 2017 Apr 21;8(28):45323–45334. doi: 10.18632/oncotarget.17335

Figure 4. PI3K inhibitors decreased cell proliferation rate and cell viability of HM-1 cells.

Figure 4

HM-1 cells were seeded in culture plates and treated with various doses of BKM120 (1, 2.5, or 5 μM) or BEZ235 (10, 100, or 1000 nM). (A) The cell numbers were significantly decreased at all doses under either BKM120 or BEZ235 treatment (*p < 0.05 vs. control). (B) The Western blot analysis showed the levels of PI3K signaling downstream proteins, pAkt and p4E-BP1, were significantly reduced with increasing concentrations of either inhibitor. GAPDH was the loading control. (C) The cell viability (%) determined by MTT/WST-1 assay was significantly reduced at all concentrations for either BKM120 or BEZ235 (*p < 0.05 vs. control). The data represented the mean ± SEM.