Skip to main content
. 2017 Apr 21;8(28):45323–45334. doi: 10.18632/oncotarget.17335

Figure 6. Triple-drug combination of etoposide, cisplatin and BEZ235 resulted in more effective cell inhibition and DNA damage in HM-1.

Figure 6

HM-1 cells were seeded in plates and were either non-treated control or treated with etoposide (8.2–21.5 × 104 nM), cisplatin (7.3–9.8 × 103 nM), BEZ235 (10 nM), or combination of etoposide + cisplatin (8.2–21.5 × 104 nM + 7.3–9.8 × 103 nM), etoposide + BEZ235 (8.2–21.5 × 104 nM + 10 nM), cisplatin + BEZ235 (7.3–9.8 × 103 nM + 10 nM), etoposide + cisplatin + BEZ235 (8.2–21.5 × 104 nM + 7.3–9.8 × 103 nM + 10 nM). (A) The single- or double-drug treatments reduced HM-1 cells viability as expected. Notably, the cell viability (%) was significantly down-regulated in the triple-drug combination group (*p < 0.05 vs. control; ***p < 0.001 vs. control). (B) DNA fragmentation was significantly increased at all treatment groups, especially in the triple-drug (etoposide, cisplatin and BEZ235) combination group. The DNA fragmentation was determined using the Cell Death Detection ELISAplus Kit (*p < 0.05 vs. control; ***p < 0.001 vs. control). The data represented the mean ± SEM.