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. 2017 Apr 30;8(28):45399–45414. doi: 10.18632/oncotarget.17535

Figure 3. The stability of candidate endogenous genes was ranked using geNorm and the NormFinder algorithm.

Figure 3

(A) Selection of the most stable reference genes from a panel of 12 genes using geNorm. The geNorm program calculated the average expression stability value (M) for each gene. Starting from the least stable genes at the left, the genes were ranked according to increasing expression stability, ending with the 2 most stable genes at the right. Candidates 5 s and 18 s were the 2 most stable genes. (B) Identification of the most stable reference gene or gene combination using NormFinder. The NormFinder algorithm ranks the set of candidate normalization genes according to their expression stability in different groups. When using this algorithm, greater gene stability is indicated by a lower stability value for the individual gene. Candidate 5 s was the most stable gene.