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. 2017 May 2;8(28):45447–45458. doi: 10.18632/oncotarget.17563

Figure 6. HERG protein expression inhibition by TGF-β1 stimulation dependent of PKA activity.

Figure 6

(A) Immunoblot analysis of HERG protein expression inhibition by TGF-β1 in NMCMs. Cells were treated with TGF-β1 (5 ng/mL) for 24 h or co-treated with H89 (10 mM) and TGF-β1 (5 ng/mL). The results of quantitative densitometric analysis results are shown in the lower panel. (B) PKA activity was measured in NMCMs using a kit with TGF-β1 (5 ng/mL) for 24 h or with TGF-β1 and forskolin (10 μM). The data represent the mean ± SEM normalized with GAPDH of three separate experiments. *P < 0.05 versus control group, #P < 0.05 versus TGF-β1 group.