Figure 4. Effect of ethanol extract of Nannochloropsis oceanica on the LPS-induced neuroinflammation.
Staining was performed in 20 μm-thick sections of mice brain. Confocal microscope observation was performed as described in the Methods section. Immunostaining of GFAP (green) and Aβ1−42 (red) protein in the hippocampus was performed with specific primary antibodies, and fluorescence was developed using Alexa 488-conjugated anti-goat and Alexa 568-conjugated anti-rabbit secondary antibodies (A). IBA-1 (red) and Aβ1−42 (green) protein in the hippocampus was performed with specific primary antibodies, and fluorescence was developed using Alexa 488-conjugated anti-mouse and Alexa 568-conjugated anti-rabbit secondary antibodies (B). Similar patterns were observed in five mice brains.