Figure 5. Effect of ethanol extract of Nannochloropsis oceanica on the LPS-induced oxidative stress in the mouse brain.

Intracellular superoxide radical production was measured by dihydroethidium in the brain. The brain sections were double stained with DHE (red) and DAPI staining (blue) (A). MDA (B) and GSH level (C) were assessed by using a specific detection kit as described in Methods. Values measured from each group of mice were calibrated by amount of protein and expressed as mean ± S.D. (n = 8). *, Significantly different from control group (p < 0.05). #, Significantly different from LPS-treated group (p < 0.05).