Figure 3. HSP70 inhibitors alter ATP abundance and promote cytochrome c re-distribution.
(A-D) The indicated melanoma tumor cell lines were treated for 48 h with various concentrations of PES, PET-16, G-TPP, Cyclo-225 or TPPO, and cellular ATP abundance was assayed. Results shown are the mean ± SD of at least three independent experiments relative to DMSO-treated control cells (*P<0.05, **P<0.005). (E) Western blot analysis for the indicated proteins extracted from mitochondria or cytosolic fractions of A875 cells treated for 24 h with 20 μM PES or PET-16.