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. 2017 Apr 21;8(28):45656–45669. doi: 10.18632/oncotarget.17321

Figure 4. PES interacts with HSP70 at mitochondria and promotes p62 oligomerization.

Figure 4

(A) Western blot analysis of p62 and LC3 protein forms in cytoplasmic and mitochondrial fractions of cells treated with PES or PET-16 (20 μM). (B) H1299 tumor cells were treated with PES (20 μM) or G-TPP (2.5 or 10 μM) followed by western blot analysis for expression of p62. (C) Purified cytosolic fractions from H1299 tumor cells were treated with increasing amounts of PES (0.5-200 μM) followed by western blot analysis for expression of p62. (D) Western blot analysis of p62 in purified cytosolic or mitochondrial fractions of H1299 cells following incubation with 20 μM of chloroquine (CQ) or PES. (E) Purified cytosolic or mitochondrial extracts from H1299 tumor cells or normal mouse liver were treated with increasing amounts of PES (0.5-200 μM) followed by western blot analysis for expression of p62. (F) Western blot of proteins isolated from cytoplasmic and mitochondrial fractions of PES-treated cells was probed with anti-ubiquitin antibody.