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. 2017 Apr 21;8(28):45656–45669. doi: 10.18632/oncotarget.17321

Figure 7. HSP70 inhibitors modify mitochondrial components.

Figure 7

(A) Western blot analysis of the PABPC1, BAK and HSP90 proteins in purified mitochondrial and cytoplasmic fractions of H1299 cells after 24 h of PES or PET-16 treatment (20 μM). (B) IP-WB analysis of the HSP70 and PABPC1 interaction in H1299 or SKBR3 breast carcinoma cells. (C) RT-qPCR analysis of RNA from A875 cells before and after treatment with PES or PET-16 (20 μM, 24 h) to assess abundance of nuclear encoded HSP70 and PABPC1 as well as several mitochondrial-encoded RNAs, as indicated. Values shown reflect mean±SD of technical triplicates. *P<0.05, **P<0.005.