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. 2017 Apr 24;8(28):45710–45724. doi: 10.18632/oncotarget.17391

Figure 4. Over-expression and knock-down of S100A15 in CL1-0 and CL1-5 lung cancer cell lines, respectively, lead to changes in proliferative, migration, and invasive properties.

Figure 4

Enhanced S100A15 expression increased proliferation of CL1-0 cells. The cell proliferations of the control and S100A15-overexpressed CL1-0 cells were determined by (A) MTT and (B) BrdU incorporation analysis after 72 h of incubation. (D) Enhanced S100A15 expression increased migration of CL1-0 cells, as determined by (C) trans-well and (D) wound healing analysis after 24 h of incubation, and (E) increased invasion of CL1-0 cells. Knockdown of S100A15 decreased proliferation of CL1-5 cells, as determined by (F) MTT and (G) BrdU assays. Knock-down of S100A15 decreased migration of CL1-5 cells as determined by (H) trans-well and (I) wound healing analysis, and (J) decreased invasion of CL1-5 cells. The migratory and invasive cells were quantified by fluorescence dye staining. All experiments were performed independently at least three times.