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. 2017 Apr 26;8(28):45898–45917. doi: 10.18632/oncotarget.17438

Figure 2. ΔRAF-1/ERTam and myr-AKT/ERTam restores EGFR downstream signaling in cetuximab treated cells.

Figure 2

A431-ΔRAF-1/ERTam- (A) and A431-myr-AKT/ERTam (B) cells were incubated with 4-OHT, EGF (10 ng/ml) or cetuximab (1 μg/ml). (A) In the absence of 4-OHT, phosphorylation of EGFR and ERK was strongly induced by EGF. Cetuximab inhibited the ligand induced activation of EGFR downstream signaling. Upon pre-incubation with 4-OHT phosphorylation of ERK1/2 as marker of MAPK signaling was strongly induced, independently of incubation with EGF or cetuximab. (B) In the absence of 4-OHT, phosphorylation of EGFR and AKT/ERTam was strongly induced by EGF. Cetuximab inhibited the ligand induced activation of EGFR downstream signaling. Upon pre-incubation with 4-OHT phosphorylation of AKT/ERTam as marker of PI3K/AKT signaling was strongly induced, independently of incubation with EGF or cetuximab.