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. 2017 Apr 28;8(28):45951–45964. doi: 10.18632/oncotarget.17504

Figure 1. The effects of CpG-HH2 complex in vitro.

Figure 1

(A) Human PBMCs (5×105) were stimulated with CpG, HH2 or CpG-HH2 adjuvant complex for 24 h. Then the supernatants were collected and used for cytokines detection by ELISA. Error bars represent mean + SEM. *p < 0.05, **p < 0.01. (B) BMDCs were incubated with Alexa Fluor 488-conjugated native NY-ESO-1 (green) alone or protein adjuvant complex for 3 h. Cells were subsequently fixed, stained with DAPI (blue) and detected by confocal laser microscopy. (C) Western blot analysis of p-p65, p-p38, and p-Erk1/2 expression.