Figure 6. The anti-tumor effect of DHA on Cal-27 cell xenograft tumor in mice.

(A) DHA noticeably inhibited the growth of Cal-27 xenograft tumor. Nude mice were inoculated with 1×10⁷ of Cal-27 cells. After the formed tumor was palpable, mice were randomly divided into two groups. DHA (25 mg/kg) was given to the ‘DHA’ group once daily for five consecutive days per week for 21 d. Six xenografts were performed in each group. The volume and weight of tumor xenografts were presented when the mice were sacrificed. Tumor diameter was measured when the mice were sacrificed. Tumor volume was calculated by the formula: V (mm³) = width² (mm²) × length (mm) ×0.5. Data are means ± SD, *P < 0.05 vs. control. The body weight changes of tumor-bearing mice at the 1^st and 21^th days. (B) Histological findings of the kidney were determined by H&E stain (upper panels) and Masson stain (lower panels) when the tumor-xenograft mice were sacrificed. Magnification × 400.