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. 2017 May 2;8(28):46006–46019. doi: 10.18632/oncotarget.17545

Figure 4. Identification of miRNAs which directly act on UTR-1.

Figure 4

(A) Alignment of the UTR-1 sequences and corresponding 3′UTR sequences from cattle and buffalo. The UTR-1 sequences are from the segment of buffalo PRNP 3′UTR (g.958-1020), corresponding to cattle PRNP 3′UTR sequences g.966-1000. (B) Illustration shows miR-338-3p and miR-145-5p binding sites for UTR-1 and corresponding mutant binding sites for UTR-1m. (C) Luciferase assays show the effects of miR-338-3p and miR-145-5p on the reporter constructs containing the UTR-1 fragment. The UTR-1 or UTR-1m construct was cotransfected into 293T cells with vectors expressing miR-338-3p or miR-145-5p or miR-neg. Luciferase assay data is presented as the mean + SD from three separate experiments. * p < 0.05; ** p<0.01.