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. 2017 May 8;8(28):46034–46046. doi: 10.18632/oncotarget.17682

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Copyright: © 2017 Jeong et al.

This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.

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Effect of CNOT2 depletion on ATG5 was examined in H1299 cells transfected by CNOT2 siRNA at mRNA (A) and protein levels (B) by RT-PCR and Western blotting. Quantitative analysis for mRNA and protein levels for ATG5 in CNOT2-depleted H1299 cells (means ± SD of 3 independent experiments. (C) Effect of HA-CNOT2 and/or HA-ATG5 on p62 degradation in MEF cells transfected with Flag-p62 plasmid. MEF cells were transfected with HA-CNOT2 and/or HA-ATG5 and/or Flag-p62 plasmids for 24 h and then Western blotting was performed. (D) The crucial role of ATG5 in p62/SQSTM1 degradation by CNOT2 depletion in ATG5 wild and mutant MEF cells co-transfected with HA-CNOT2 and Flag-p62 plasmids by Western blotting.