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. 2017 Apr 29;8(28):46065–46070. doi: 10.18632/oncotarget.17521

Figure 2.

Figure 2

(A, B) Immunoblot analysis of P-ERK and total ERK in whole cell lysate obtained from patient lesion (A) or HEK293 cells transiently transfected with plasmids expressing either wild type or p.D321N ERK2 protein (B). GAPDH served as a loading control in ‘B’. (C) Immunoblot analysis of P-ERK and total ERK in whole cell lysate obtained from the patient lesion treated for 4 hours with either 200 nM of the BRAF-V600E inhibitor vemurafenib, or 10 nM of the MEK inhibitor trametinib (Selleckchem, Houston, TX), or 40 nM of the ERK inhibitor TCS ERK 11e (Tocris Bioscience, Bristol, UK).