Skip to main content
. 2017 May 2;8(28):46121–46135. doi: 10.18632/oncotarget.17555

Figure 1. MNK inhibition regulates the phosphorylation and overall expression of NDRG1 in MDA-MB-231 breast cancer cells.

Figure 1

Breast cancer cells were treated for different times with MNK-I1 (5 μM). Levels of specific proteins in (A) MDA-MB-231 and (B) MDA-MB-453 cells were analysed by Western blot with indicated antibodies (the latter display two distinct bands for P-eIF4E). Ratio of p-NDRG1/NDRG1 was calculated using Image J. NDRG1 mRNA levels in (C) MDA-MB-231 and (D) MDA-MB-453 cells were analysed by RT-qPCR. (E) MDA-MB-231 cells were pretreated with MNK-I1 (5 μM) for 4 h and followed by 100 ng/ml actinomycin D for 16 h, where indicated. NDRG1 mRNA were analysed by qPCR. Data are shown as mean ± S.E.M. from three replicates. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.