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. 2017 May 2;8(28):46163–46176. doi: 10.18632/oncotarget.17575

Figure 2. Effect of 5azadC treatment on cellular transcription factor expression.

Figure 2

(A) Total RNAs were extracted from Ca Ski and SiHa cells treated or not with 5azadC for 96h and the relative expression of Oct1, TEF-2, NF1, YY1, cFos, cJun, CEBPβ and Sp1 mRNA was measured by RT-qPCR and normalized to β2M mRNA level. The data are presented as mean values from at least three independent experiments. Error bars represent the standard deviation and p values were calculated by performing Mann-Whitney test: NS: no significant. (B) Mock treated and 5azadC-treated (0.25 μM for 96h) Ca Ski and SiHa cells were lysed and the protein fraction was assayed for the presence of Sp1 by Western blotting. β-actin served as a loading control. A representative blot is shown; the values of the densitometric analysis were normalized with β-actin (left). The data are presented as mean values from at least three independent experiments. Error bars represent the standard deviation and p values were calculated by performing Mann-Whitney test: NS: no significant (right).