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. 2017 May 2;8(28):46163–46176. doi: 10.18632/oncotarget.17575

Figure 4. Destabilization of E6 transcripts after 5azadC treatment.

Figure 4

(A, B) RNAs were extracted from Ca Ski (A) and SiHa (B) cells treated or not with 5azadC at 0.25 μM during 96h and treated with 4 μg/mL actinomycin D. Relative expression of E6Fl mRNA was determined by RT-qPCR and normalized to β2M mRNA level. The diagram shows the mean values for at least three independent experiments and the standard deviation. Mann-Whitney`s test was performed to calculate p values using DMSO treated cells as a reference control. *p < 0.05, **p < 0.01.