Table 1. Primer sequences.
Gene | Forward (5′->3′) | Reverse (5′->3′) | Amplicon |
---|---|---|---|
E6Fl | CACAGGAGCGACCCAGAAA | CCCGAAAAGCAAAGTCATATACC | 131 bp |
E6*I | CTGCGACGTGAGGTGTATTA | TGTCCAGGTGTCTTTGCTTT | 68 bp |
E6all | GAGAACTGCAATGTTTCAGGACC | TGTATAGTTGTTTGCAGCTCTGTGC | 80 bp |
B2M | GATGAGTATGCCGTGTG | CAATCCAAATGCGGCATCT | 114 bp |
Oct1 | CAGATCGCACAGGATCTTCA | GGATGCACCAACACAAACTG | 80 bp |
TEF-2 | AAAGCTCCCACTTGAAAGCA | AACTTCCATGTGCACCCTTC | 85 bp |
NF1 | GTGTTCCATGTGCGATTACG | TCATGTGCCTTTTCAGCTTG | 140 bp |
YY1 | ACCTGGCATTGACCTCTCAG | TTCTCATGGCCGAGTTATCC | 149 bp |
cFos | AGAATCCGAAGGGAAAGGAA | CTTCTCCTTCAGCAGGTTGG | 150 bp |
cJun | TGACTGCAAAGATGGAAACG | CAGGGTCATGCTCTGTTTCA | 119 bp |
CEBPβ | TACTACGAGGCGGACTGCTT | AGGTACGGGCTGAAGTCGAT | 149 bp |
U6 | CGCTTCGGCAGCACATATAC | AAAATATGGAACGCTTCACGA | 100 bp |