Table 2. List of 26 top proteins selected in the discovery phase.

Clinical scenario considered	27 cases vs 13 controls		10 metastatic vs 13 controls		17 non-metastatic vs 13 controls		10 metastatic vs 17 non-metastatic
	(486 proteins considered)		(335 proteins considered)		(389 proteins considered)		(437 proteins considered)
protein	p-value logistic model	p-value Fisher	p-value logistic model	p-value Fisher	p-value logistic model	p-value Fisher	p-value logistic model	p-value Fisher
Fibrinogen alpha polypeptide isoform alpha-E preprotein	0.01	0.01	<0.001	<0.001	0.05	0.06	0.07	0.09
Pancreatic ribonuclease precursor	0.28	0.39	0.09	0.13	0.71	1	0.11	0.15
Fibrinogen. gamma chain isoform gamma-A	0.03	0.03	0.94	0.01	0.18	0.26	0.95	0.12
Inter-alpha (globulin) inhibitor H4	0.08	0.07	0.02	0.02	0.27	0.35	0.07	0.10
Alpha 1 type XVIII collagen isoform 1 precursor	0.1	0.12	0.95	0.05	0.38	0.44	0.96	0.26
Latent transforming growth factor beta binding protein 2	0.85	1	0.71	1	0.98	1	0.71	1.00
Insulin-like growth factor binding protein 4	0.96	0.04	0.95	0.01	0.96	0.24	0.09	0.10
Insulin-like growth factor binding protein 6	0.28	0.39	0.09	0.13	0.71	1	0.11	0.15
Histidine-rich glycoprotein precursor	0.96	0.04	0.95	0.01	0.96	0.24	0.09	0.10
Procollagen C-endopeptidase enhancer	0.96	0.02	0.96	0.07	0.95	0.02	0.78	1.00
Alpha 1 type I collagen preprotein	0.95	<0.001	0.94	<0.001	0.95	0.02	0.22	0.26
Melanoma inhibitory activity	0.15	0.23	0.09	0.13	0.27	0.35	0.37	0.42
Plasma kallikrein B1	0.96	0.28	0.96	0.07	0.98	1	0.12	0.13
Neural proliferation, differentiation and control. 1	0.81	1	0.41	0.62	0.77	1	0.25	0.33
Alpha 2 type I collagen	0.95	<0.001	0.94	<0.001	0.95	0.02	0.22	0.26
Complement factor D	0.96	0.15	0.96	0.07	0.97	0.49	0.25	0.33
Elastin isoform a precursor	0.96	0.15	0.96	0.07	0.97	0.49	0.25	0.33
Seleprotein P isoform 1	0.38	0.64	0.09	0.13	0.84	1	0.05	0.05
Matrix Gla protein	0.28	0.39	0.09	0.13	0.71	1	0.11	0.15
GDNF family receptor alpha 2 preproprotein	0.81	1	0.2	0.34	0.41	0.56	0.05	0.05
Immunoglobulin superfamily, member 8	0.33	0.37	0.71	1	0.96	0.07	0.96	0.04
Matrix metalloproteinase 14 preproprotein	0.97	0.54	0.97	0.18	0.98	1	0.29	0.54
Complement factor H-related 3	0.97	1	0.97	0.18	.	.	0.97	0.13
ST6 beta-galactosamide alpha-2,6-sialyltranferase 2	0.96	0.28	0.96	0.07	0.98	1	0.12	0.13
Mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase	0.97	1	0.97	0.18	.	.	0.97	0.13
Somatostatin preproprotein	0.97	0.54	0.97	0.18	0.98	1	0.29	0.54