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. 2017 May 3;8(28):46191–46203. doi: 10.18632/oncotarget.17580

Figure 4. NDN directly binds to the GN box in the promoter of LRP6 in CRC cells and suppresses the Wnt signaling pathway.

Figure 4

(A) ChIP assay showed a potential binding region (5F to 5R, -190bp to 0bp) of NDN in LRP6 promoter. (B) Expression of LRP6 in the indicated cells. (C) The Wnt signaling luciferase reporter assay of the indicated cells transfected with NDN or shNDN vector, respectively. (D) Western blot assay for β-catenin in cytoplasm and nucleus of the indicated cells. LamB1 and β- tubulin served as loading controls for nuclear and cytoplasmic proteins. Error bars represent the means ± SD from three independent experiments, * p<0.05, ** p<0.01.