Table 1.

Lysis times and interval between stop of rotation and actual cell lysis

S allele†	Time from induction until lysis in broth culture, min‡	Experiment 1		Experiment 2
		Time from induction until lysis for tethered cells, min§	Time from rotation stop until lysis, sec¶	Time from induction until lysis for tethered cells, min§	Time from rotation stop until lysis, sec¶
Sam7	No lysis	No lysis	No lysis	No lysis	No lysis
S	45			104  ± 8 (15)	19  ± 6 (15)
S105	40	64  ± 7 (15)	13  ± 4 (15)
SA52G	10	19  ± 2 (5)	26  ± 7 (5)
S105A52G	15	25  ± 3 (20)	32  ± 9 (20)
SC51S	10			27  ± 4 (15)	40  ± 6 (15)
S105C51S	15	27  ± 1 (23)	26  ± 7 (23)	31  ± 4 (15)	39  ± 8 (15)

^†  The motile strain RY8797pQ carrying pS105 or isogenic derivatives of this plasmid was used in all experiments. 

^‡ Cells were cultured in LB medium at 37°C. At an A550 of 0.2, expression of the lysis genes was induced by the addition of IPTG and arabinose. The turbidity of the culture was followed until the A550 dropped below 0.05. The time when the turbidity of the culture started to decline was defined as the lysis time. 

^‡ Cells were grown and induced as described in Materials and Methods and tethered to glass coverslips. The time until lysis for individual cells was determined by microscopic observation. The number of cells analyzed is shown in parentheses. 

^¶ Time between the halt of rotation and lysis of tethered cells.