Table 2.

Interval between the stop of rotation and lysis as a function of endolysin content

Plasmids†	Time from induction until lysis for tethered cells, min‡	Time from rotation stop until lysis, sec§
pS105A52G	25.1  ± 2.0 (15)	37.9  ± 9.9 (15)
pZA32-luc
pS105A52GΔR	27.1  ± 2.7 (15)	11.5  ± 3.1 (15)
pZA32-R/Rz/Rz1
pS105A52GΔR	27.5  ± 2.0 (15)	12.8  ± 4.4 (15)
pZA32R

^† The indicated plasmids were introduced into strain RY8797pQ. Transformants carrying the three plasmids were grown, tethered to glass coverslips, and induced with IPTG and arabinose as described in Materials and Methods. 

^‡ Lysis times for individual cells were determined; mean values are shown. The number of individual cells analyzed is given in parentheses. 

^§ Time after rotation stop until cell lysis.