Table 2. Limit of detection for beef and pork DNA in dddPCR assays (n = 36).
ng/μl | ng/tube | BP | BN | DR | PP | PN | DR |
---|---|---|---|---|---|---|---|
10 | 40 | 36 | 0 | 100% | 36 | 0 | 100% |
5 | 20 | 36 | 0 | 100% | 36 | 0 | 100% |
2.5 | 10 | 36 | 0 | 100% | 36 | 0 | 100% |
1 | 4 | 36 | 0 | 100% | 36 | 0 | 100% |
0.5 | 2 | 36 | 0 | 100% | 36 | 0 | 100% |
0.1 | 0.4 | 36 | 0 | 100% | 35 | 1 | 97.20% |
0.05 | 0.2 | 28 | 8 | 77.80% | 24 | 12 | 66.70% |
0.01 | 0.04 | 18 | 18 | 50% | 0 | 36 | 0% |
0.005 | 0.02 | 0 | 36 | 0% | 0 | 36 | 0% |
BP = Beef Positive, BN = Beef Negative, PP = Pork Positive, PN = Pork Negative, DR = Detection Rate.