Fig 6. Exogenous irisin administration downregulated PGC-1 alpha, myostatin-a and b mRNA expression and upregulated troponin C and T2D mRNA expression in zebrafish.

Exogenous irisin (1 ng/g and 10 ng/g B.W) significantly downregulated PGC-1 alpha (A) relative mRNA expression in zebrafish heart and skeletal Irisin (0.1 and 1 ng/g B.W) upregulated troponin C mRNA expression (B) in zebrafish heart and skeletal muscle when compared controls. However, exogenous irisin (0.1 ng/g 1 ng/g and 10 ng/g B.W) downregulated myostatin-a relative mRNA expression in zebrafish muscle and heart (C). Upon exogenous administration of irisin at 0.1 ng/g and 1 ng/g B.W, troponin T2D relative mRNA expression was downregulated in zebrafish heart and skeletal muscle (D). Also, irisin (0.1 ng/g, 1 ng/g and 10 ng/g B.W) downregulated myostatin-b relative mRNA expression in zebrafish when compared to saline treated controls (E). No significant effect was observed in response to i.p injection of irisin towards beta-actin relative mRNA expression in heart and muscle of zebrafish (F). Asterisks denote significant differences between control (saline group) and irisin, siRNA injected groups of the same (* p<0.05, n = 8 fish/group). Data are represented as mean + SEM. One-way ANOVA followed by Tukey’s multiple comparison post hoc test were used for statistical analysis.