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. 2017 Aug 3;12(8):e0181461. doi: 10.1371/journal.pone.0181461

Fig 7. Knockdown of irisin by siRNA altered the expression of mRNAs encoding cardiac muscle proteins in zebrafish.

Fig 7

In vivo administration of irisin siRNA (10 ng/g B.W) downregulated PGC- 1 alpha (A) mRNA expression in zebrafish heart and skeletal muscle samples when compared to control group. However, irisin siRNA (10 ng/g B.W) significantly upregulated myostatin a (B) and myostatin b (C) relative mRNA expression in heart and muscle tissues of zebrafish when compared to control group (B, C). In addition to that, knockdown of irisin by siRNA downregulated troponin C and troponin T2D relative mRNA expression in zebrafish heart and skeletal muscle when compared to saline treated controls (D, E). No significant effect on PGC- 1 alpha, troponin C, troponin T2D, myostatin a and b relative mRNA expression was observed when scrambled siRNA was injected (A-E).