Table 1. Forward and reverse primers, and the annealing temperature used in PCR and qRT-PCR analyses of the expression of mRNAs of interest.

Gene	Primer sequence (5’-3’)		Annealing temperature (°C)
	Forward	Reverse
FNDC5b	GCTTATATCTTCGCGTCCTC	GCCAGTTTCTCTGACTCTTT	59
PGC- 1 alpha	TCTATTCGGAAGGGCCCAGA	GGTGGTGCTGTCTCGTTTTG	58
Myostatin-b	TCCTTTAGCACGCCTTGGAA	TGCTTGAGTCGGAGTTTGCT	60
Myostatin-a	TTTTGAGCATCCTGCGCCTA	ATCTTTGGGCTCAGTGCGAA	60
Troponin-C	GCAGAAAAATGAGTTCCGTGC	TTCCGCCAGTTCTTCCTCTG	60
Troponin T2D	AGTTCAGGAGGAAGTGGATGAGT	AGTCTGGCTTGACGCTCTTTC	60
β-Actin	CTACTGGTATTGTGATGGACT	TCCAGACAGAGTATTTGCGCT	59
18s	GGATGCCCTTAACTGGGTGT	CTAGCGGCGCAATACGAATG	60