Table 1. Comparison of the purification yields of scFv-h3D6-Ec and scFv-h3D6-Pp.
Values were obtained from the purification of 1L of initial growth culture. Values are presented as mean±SD.
| scFv-h3D6-Ec | scFv-h3D6-Pp | ||
|---|---|---|---|
| Purification step | Total protein (mg) | Purification step | Total protein (mg) |
| Insoluble fraction | 119.9±0.2 | Medium (48h expression) | 337.8±9.2 |
| AS Supernatant | 120.0±2.5 | ||
| After refolding/Before CEX | 81.1±4.9 | AS Pellet/Before CEX | 206.4±18.8 |
| Scrambled fraction | 4.0±0.1 | ||
| Native protein | 2.9±0.2 | Pure protein | 20.5±1.8 |