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. 2017 Aug 3;15(8):e2002267. doi: 10.1371/journal.pbio.2002267

Fig 2. Excess FliP bypasses the requirement of FliO for flagellar-specific type III secretion system (fT3SS) function and motility.

Fig 2

(A) Flagellin secretion in the wild-type (WT) (EM2320) and the ΔfliO mutant (EM2323, EM2324) harboring an empty (vector control [VC]) or fliP (pfliP)-expressing vector. Complementation was induced by the addition of 0 μM, 1 μM, and 5 μM sodium salicylate (NaSal). Secreted flagellin was detected by Western blot using anti-FliC antibodies. DnaK was used as loading control. (B) Motility of the WT and the ΔfliO mutant complemented with the empty (VC) or fliP (pfliP)-expressing vector in 0.3% swim agar plates after 5 h at 37°C. Expression of fliP was induced using 5 μM NaSal. Relative motility reports the mean ± SD, n = 4.