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. Author manuscript; available in PMC: 2018 Jul 11.
Published in final edited form as: Biochemistry. 2017 Jun 28;56(27):3422–3433. doi: 10.1021/acs.biochem.7b00325

Figure 4.

Figure 4

Cooperative folding can be induced through crowder-driven secondary structure destabilization. First-derivative curves of thermal denaturation experiments of FL tRNA and SSS with increasing concentrations of (upper row) PEG200 and (lower row) PEG8000 in (A) 0, (B) 0.5 and (C) 2.0 mM Mg2+, with increasing concentrations of PEG200 or PEG8000 from 0% to 20% to 40% (w/v). The TM’s are provided in Tables S1, S2 and S3. Solutions contain a background of 10 mM sodium cacdoylate (pH 7.0) and 140 mM KCl.