Skip to main content
. 2017 Aug 3;12(8):e0181738. doi: 10.1371/journal.pone.0181738

Fig 5. Animals mount T cell responses against the H1N1pdm challenge after vaccination with H5 mosaic MVA vaccine.

Fig 5

We detected T cell responses in peripheral blood mononuclear cells (PBMC) using an IFN-γ elispot assay. PBMCs were stimulated with 7 pools of overlapping synthetic peptides from HA. We detected no responses 7 days after prime (A) or 14 days after boost (B). T cell responses were detected 7 days after challenge, with most responses detected in PBMCs stimulated with peptide pool 6 (C). The H5 mosaic HA used in vaccination had the most amino acid sequence identity shared with the challenge strain A/California/04/2009 (H1N1pdm) in the HA 2 domain, with the highest sequence identity of 82.4% occurring within peptide pool 6 (identical amino acid residues indicated by a black stripe; D). Error bars in A, B, and C indicate standard deviation.