Fig 5. IL-25 induced activation of MAPKs and NF-κB in NPDF.

Effects of IL-25 on the activation of mitogen-activated protein kinases (ERK, p38, JNK) and NF-κB evaluated by Western blotting. Phosphorylation of representative MAPKs, ERK (A), JNK (B), and p38 MAPK (C) was induced by IL-25 treatment, after which they became inhibited by their specific inhibitors, including U, SP, and SB, respectively. (p < 0.05). IL-25 induced phosphorylation of IκBα; p-IκBα expression was inhibited by Bay (p < 0.05) (D). Values are expressed as means ± standard errors of independent experiments. (*p < 0.05 vs control). †p <0.05 vs IL-25 alone. Triple tests were performed on all of the experiments. U = specific inhibitor of ERK, SB = specific inhibitor of p38, SP = specific inhibitor of JNK, Bay = specific inhibitor of U = specific inhibitor of NF-κB.