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A, Expression of the GPR120 gene. Lane 1, maker; lane 2, wild-type (WT) mouse; lane 3, klotho mutant (kl/kl) mouse and lane 4, C57/BL6 mouse (positive control). B, GPR120 downregulation by siRNA. Lane 1, si-GPR120 RNA and lane 2, s-scramble RNA. C, Effects of GPR120 gene knockdown on NOX4 gene expression and NOX activity in arterial SMCs of kl/kl mice (n = 6, each).
