Fig 2. Acute effects of treadmill exercise on the mRNA expression of known exercise-responsive genes.
The soleus muscles of exercised C57BL/6 PAD mice (n = 4 per time point, total 24, left panels) and KK-Ay PAD mice (n = 6 per time point, total 30, right panels) were obtained before and after the fifth treadmill exercise session. The mRNA expression levels of Pgc1a (A), Il6 (B), Nr4a1 (C), Nr4a2 (D), and Nr4a3 (E) in the soleus muscles were determined by qRT-PCR, normalized to Rplp0 expression level, and calculated relative to the pre-exercise group (0 min). The results are expressed as the mean ± SD. *p < 0.05, **p < 0.01 vs. 0 min (Dunnett’s test). Bold bars represent the timing of the fifth treadmill exercise.